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In 2009, PacBio published a paper expanding the principle into a full-blown sequencing technique. Once again, each nucleotide was labeled with a different colored fluorophore detectable by the ZMW to determine which base had been incorporated. The fluorophores were attached such that they would be cleaved off during the chemical reaction incorporating the base into the growing DNA strand; they would then diffuse out of the ZMW so that the next fluorophore could be detected. Sequencing took place on a chip with many wells simultaneously — a different type of parallelization where each well detected a single DNA molecule undergoing the same basic chemical reaction.
。业内人士推荐体育直播作为进阶阅读
What surprised me was that this entire walk is fully hardware-driven -- no microcode involvement at all. The state machine reads the page directory entry, reads the page table entry, checks permissions, and writes back the Accessed and Dirty bits, all autonomously. Since it's hardware-driven, it runs in parallel with the microcode and needs its own memory bus arbitration -- the paging unit must share the bus with both data accesses from the microcode and prefetch requests from the instruction queue.
AdjustLink sticks a list around MultiLink, using features